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This book will once again serve as a first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. Covering methods for microbes representing all three domains of life--Bacteria, Archaea, and Eukarya--this manual should be on the bookshelf of every serious practitioner of microbiology in academia, industry, and government, and in clinical laboratories. It will serve as a rich resource on methods for seasoned professionals as well as for students at all levels.
Many of the new chapters cover important topics such as molecular genetics of archaea; molecular genetic manipulations using phages as tools; state-of-the-art technology used in studying complex, naturally occurring microbial communities; metabolism of natural polymers including cellulose, hemicellulose, and lignin; DNA microarray technology; physiology, metabolism, molecular genetic approaches to the study of filamentous fungi, and general and molecular approaches for studying microbial symbioses.
Each section details reliable methods that are widely applicable to basic studies. Additionally, chapters present background principles to facilitate understanding of the how and why of a given method, followed by a step-by-step description of the procedure. Common problems, precautions, and pitfalls of the methods are also presented. In many cases, commercial sources for equipment and materials are also given.