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        T132 Registration Fee (non-member) $45.00
        T132 Registration Fee (member) $25.00
        T132 Real-Time PCR for Nucleic Acid Amplification and Detection


        Date: January 29, 2013; 2:00 p.m. (EST)
        Speaker: Richard L. Hodinka, Ph.D.; The Children's Hospital of Philadelphia, PA

        Description: After being introduced in the mid-1990s, real-time PCR has rapidly become the “gold standard” for molecular diagnostic testing. Numerous advances in this technology over the years have lead to a variety of real-time assays and platforms to quickly and accurately identify and/or characterize many different microbes of medical importance.

        Intended Audience: The course is open to anyone! However, since it is a practical course, laboratory personnel (e.g., medical technologists, supervisors, and directors) with a minimum of a bachelors-degree and familiarity with clinical microbiology would benefit the most. Limited to U.S. and Canadian residents.

        Objectives: Following this session, the participants should be able to:

        • Discuss the similarities and differences between conventional end-point PCR and real-time PCR.
        • Describe the principles of real-time PCR and the parameters needed to successfully perform this method.
        • Compare and contrast the molecular instruments and technologies for real-time amplification and detection.

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